Imaging and Microscopy Centre

Confocal and super resolution microscopes

Confocal, super-res and widefield room

Cryostat and Flow Cytometer

More Information

About the Imaging and Microscopy Centre

The Imaging and Microscopy Centre is a core facility for advanced life sciences imaging at Southampton University.

We cater for a broad range of research requirements, from internal university users to industry and other institutions.

Facilities and equipment

Types of equipment provided by the IMC:

We provide image analysis software for 2D and 3D datasets. You can book our equipment, big-data handling, image analysis and training. We can also supply quotations and assistance for grant applications.

Technical specification

Flow cytometry

We have a flow cytometry machine for measuring cells in solution.

Millipore Guava

Sample format

Single 1.5 ml Eppendorf tube

Filters

Excitation laser and Emission

Blue 488 nm (nanometer), green 525/30 nm
Red 642 nm, yellow 583/26 nm
red 661/15 nm
red 695/50 nm
forward Scatter
side Scatter

Western blotting

We have western blotting equipment for detecting specific proteins in tissue samples

LiCor Odyssey NIR fluorescence flatbed scanner

Detectors

2 x Photomultiplier tube

Lasers and filters

Excitation laser λ and emission filters

685 nm - 700 nm LP (Longpass filters)
785 nm - 800 nm LP

Microinjection

We have microinjection equipment for precision injection very small amounts of fluid into biological cells or nuclei

Eppendorf femtojet electronic microinjection system

Mounted on Zeiss Axiovert 200 stand

Analysis software

Industry-standard image analysis software packages are provided installed on a high-spec analysis PC which can be reserved via the IMC booking calendar:

Imaris – 3D image analysis software

FlowJo – flow cytometry analysis software.

Matlab

FIJI

Expert team

We organise seminars and workshops free-of-charge for all users of facility equipment.
We can also arrange one-to-one or group training in experimental design for any technique.

Mariana Vargas-Caballero is an expert in electrophysiology (patch clamp and field recording), and will train users and assist with experimental design.

Mark Willett is an expert in all forms of fluorescence imaging and can offer support with training and experimental design.

The facility works in close collaboration with the Photonics lab headed by Prof Sumeet Mahajan.

Please get in touch with one of the team to find out more.

Facility manager and IMC Highfield: Dr Mark Willett m.willett@soton.ac.uk

Electrophysiology IMC Highfield: Dr Mariana Vargas-Caballero M.Vargas-Caballero@soton.ac.uk

IMC Southampton general Hospital: Ms Georgina Dawes G.R.Dawes@soton.ac.uk

Academic lead for imaging: Dr Katrin Deinhardt

Dr Mark Willett

Exp Officer Imaging Facilities Mgr

Connect with Mark

Email: M.Willett@soton.ac.uk

Dr Mariana Vargas-Caballero

Associate Professor

Connect with Mariana

Email: M.Vargas-Caballero@soton.ac.uk

We specialise in advanced imaging techniques. Our expertise also extends to histological microscopy and electrophysiology. Whether you are an internal or external user from the academic or industrial sector, the centre is open to all.

Mark Willet

Imaging Facilities Manager