Fluorescence and brightfield microscopes

More Information

About the Fluorescence and brightfield microscopes

We have a range of widefield and fluorescence miscroscopes.

We have equipment at both Highfield and Southampton General Hospital.

Part of: Imaging and microscopy centre

Technical specification

Our widefield and zoom fluorescence microscopes permit the specific labelling of up to four targets simultaneously. We also offer conventional brightfield microscopy to very high magnifications, or on our zoom microscope.

Olympus IX83 Widefield epifluorescence microscope

  • epifluorescence - 250 nm resolution
  • phase contrast - 250 nm resolution

Objectives

Magnification, numerical aperture and immersion
  • 4x, 0.13, air
  • 10x, 0.5, air
  • 20x, 0.7, air
  • 40x, 0.9, air
  • 63x, 1.42, oil
  • 100x, 1.49, oil
Specimen format
  • standard glass slide
  • 35 mm coverslip bottom dish
  • coverslip bottom chamber-slide
  • optical bottom multi-well plate
Detector

Hamamatsu Orca Flash4.0 sCMOS. 2048 x 2048 pixels.

Filtersets

Expansion, electron microscope and colour

  • 350/50 nm, 460/50 nm, Blue
  • 470/40 nm, 525/50 nm, Green
  • 545/30 nm, 620/60 nm, Orange
  • 560/40 nm, 630/75 nm, Red
  • 620/60 nm, 700/75 nm, Far Red
Other features
  • transmissed light - yes
  • DIC - no
  • environmental temperature/gas control - no
  • hardware autofocus - no
  • simultaneous acquisition - no
  • sequential fluorescence acquisition - Up to 5 channels
  • mark and find - yes
  • sequential fluorescence acquisition - Up to 5 channels
  • time lapse - yes
  • photo-knetics - no
  • 3D imaging - no

M205 FCA fluorescence/brightfield Zoom Stereomicroscope

Modalities
  • epifluorescence
  • transmitted light base
  • reflected light (ring illumination)
  • reflected light (goose necks)
Magnification

7.8-160x with coded readout/automatic image scaling

Emission filters
  • blue
  • green
  • red
Camera

Colour/monochrome CCD 1280 x 960 pixels

Hirox HRX-01 digital multi-modal zoom/compound microscope

This is an excellent microscope for the following techniques:

Protein crystal imaging

High resolution full-colour 2D and 3D imaging of environmental science, micro-zoology, palaeontological and archaeological specimens.

  • very high resolution 2D/3D label-free imaging.
  • ring light
  • transmitted motorized light stage
  • 3D Z-scanning
  • 3D Photogrammetry attachment
  • polarised contrast filtering

Leica DM4B Upright, motorised fluorescence/Brightfield microscope

Magnification, numerical aperture and immersion
  • 2.5x, 0.7, air
  • 5x, 0.15, air
  • 10x, 0.30, air
  • 20x, 0.40, air
  • 40x, 0.65, air
  • 63x. 0.80, air 
Other features
  • live Imaging chamber – No
  • DIC – No
  • stage – Manual
  • camera – CCD colour and monochrome
  • filter sets – DAPI, FITC, TRITC

Leica DM5000B Upright, motorised fluorescence/brightfield microscope

Magnification, numerical aperture and immersion
  • 10x, 0.25, air
  • 20x, 0.5, air
  • 40x, 0.75, air
  • 63x, 1.75, oil
  • 100x, oil
Other features
  • live Imaging Chamber – no
  • DIC - no
  • stage - manual
  • camera - CDD colour and monochrome
  • filter sets - DAPI, FITC, TRITC 

Contact us

Dr Mark Willett - Highfield
Georgie Dawes - Southampton General Hospital

Highfield

Building 85, Highfield Campus, Southampton SO17 1BJ
We’re open Monday to Friday 09:00 to 17:00 UK time.

Hospital

Southampton General Hospital, Tremona Rd, Southampton SO16 6YD