Project overview
The aim of this project was to develop the first, tightly controlled saturation mutagenesis methodology for the Chlamydiae using a methodology termed TraDIS (transposon directed insertion site sequencing). Saturation mutagenesis can be used to differentiate essential from nonessential genes, which can help in the discovery of new drug or vaccine targets. Through optimisation of gene expression, site-directed mutation of the transposase gene and utilisation of long-read nanopore sequencing to identify transposon insertions, we achieved proof of principle for this important new genetic tool.
Staff
Lead researchers
Other researchers
Research outputs
Colette E O'Neill, Rachel J. Skilton, Jade Forster, David W. Cleary, Sarah A. Pearson, David J. Lampe, Nicholas R. Thomson & Ian N. Clarke,
2021, Wellcome Open Research , 6
Type: article
Rachel J Skilton, Colette O'Neill, Nicholas R Thomson, David J Lampe & Ian N Clarke,
2021, Wellcome Open Research , 6
Type: article